Isolation and cloning of a Azospirillum lipoferum locus that complements Escherichia coli proU mutant
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چکیده
منابع مشابه
Osmoregulation in Escherichia coli: complementation analysis and gene-protein relationships in the proU locus.
The proU locus in Escherichia coli encodes an osmotically inducible transport system for two substrates, glycine betaine and L-proline, whose intracellular accumulation represents an important component in the physiology of osmoregulation. Several osmoresponsive proU::lac mutants were isolated and tested for complementation with plasmids carrying different functional regions of proU. Three clas...
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Azospirillum lipoferum 4T has original properties such as nonmotility, melanin synthesis, and laccase activity. Following random Tn5 mutagenesis in A. lipoferum 4T, we obtained 10 mutants which were affected in melanization and laccase activity. The class 1 mutants, with intermediate levels of laccase activity, showed some coloration; the class 2 mutants, which were completely negative for lacc...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1998
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(98)00129-3